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Products/Services
Environmental Microbiology Services
The MiL, Inc., is an independent, full-service microbiology laboratory,
providing a broad range of microbial and biochemical analytical
services targeted to the environmental industry.
Our laboratories are staffed with skilled and internationally
recognized Ph.D. microbiologists and Ph.D. biochemists.
Lab services include the isolation, enumeration, identification
and R&D analyses of environmental microorganisms using leading
edge technologies. Consulting services are focused towards method
development and validation, bioremediation feasibility studies
and testing for soils and wastewaters, bacterial genetics, soil
nutritional analysis, specialized chemistries, and regulatory
issues.
Our diversified and worldwide customer base includes Fortune
500 companies, environmental engineering and consulting firms,
dedicated bioremediation service providers, manufacturers and
distributors of microbial cultures, marketers of nutrients and
surfactants, research groups, and local, state and federal regulatory
entities including many national laboratories.
Specific services include:
- Biofeasibility Studies
- Characterization of Biodegrading Microorganisms
- Bioremediation Monitoring
- Microcosm Treatability Testing
- Wastewater Biotreatment
- Consulting Services
Food Microbiology Services
Test services include:
isolation, enumeration, and identification of pathogenic, indicator
and spoilage organisms; challenge and shelf life studies; sanitation
audits; QA and HACCP consultation.
We are equipped to test food and food-grade products as well
as environmental surfaces and ambient air within food production
facilities. Our specialties include microbial identification and
confirmations, R&D analyses, consulting, method development
and validations, toxins, challenge and shelf life studies, sanitation
audits, QA and HACCP consultations. Overnight your food samples
and isolates in our convenient sample cooler kit, for rapid overnight
testing of pathogens such as Salmonella, Listeria, E.coli O157-H7,
Campylobacter, Shigella and Staphylococcus aureus.
Food Pathogen Testing
Some of the species we routinely test:
- Bacillus cereus
- Campylobacter
- Clostridium botulinum
- Clostridium perfringens
- Hemorraghic and Enteropathogenic E. coli
- Listeria monocytogenes
- Salmonella
- Shigella
- Staphylococcus
- Vibro species
- Yersinia enterocolitica
Microbial Identification Methods
GC-FAME (Gas Chromatograph Fatty Acid Methyl Ester)
Also known as Cellular Fatty Acid Analysis, allows for bacterial
or yeast identification based on the specific fatty acid composition
of the cell wall. Fatty acids are extracted from cultured samples
and are separated by gas chromatography. A computer generated,
unique profile pattern of the extracted fatty acids (9 to 20 carbon
chains long) is compared through pattern recognition programs,
to our microbial databases of well over 2600 species and subspecies.
Our reports include the fatty acid profiles, a listing of the
best database matches, along with an assigned statistical probability
value indicating the confidence level of the match.
Biolog® MicroLog
This automated, metabolic characterization system uses a 96-well
microtiter plate arrayed with different carbon sources and the
microorganism of interest. Each microorganism has unique capacities
to oxidize the various carbon sources. Tetrazolium dye in each
of the wells turn darker shades of purple as the carbon sources
are oxidized by the microorganism. The test yields a characteristic
pattern of positive (purple) and negative wells which provide
a metabolic signature of the inoculated organism. The system's
computer the pattern signature with the database of interest to
determine an identification. Databases include aerobes, anaerobes,
gram positive & negative bacteria, yeast, actinomycetes, and
lactic acid bacteria.
RiboPrinter™ (Qualicon™, a DuPont subsidiary)
The RiboPrinter™ system characterizes bacteria by creating
a fingerprint pattern of an isolate's genetic material. Pure colonies
are isolated from culture, inactivated by heat and loaded into
the sample carrier. DNA is extracted from the cell lysate and
is digested to completion using an EcoR1 restriction enzyme. The
DNA fragments are separated according to molecular size by gel
electrophoresis and are the transferred to moving Nylon membranes.
After denaturation, each membrane is hybridized with a chemically
labeled rRNA operon probe from E. coli. The membrane is washed
and treated with a blocking buffer and an antisulfonated, DNA
antibody/alkaline phosphatase conjugate. Unbound conjugate is
removed and a chemiluminescent substrate applied. This step makes
each electrophoresis band containing genetic information from
the rRNA genes visible to a custom CCD camera in the RiboPrinter™
system. The camera detects the light intensity of the targeted
bands and converts the patterns from luminescent DNA fragments
to digital information. Using algorithms, a pattern is extracted
from the image data and is compared to other known patterns stored
in the database to characterize and identify each sample.
click here for more info
MicroSeq™ 16S rRNA Gene Sequencing (PE Applied
Biosystems)
Genomic DNA is extracted directly from dead or alive bacterial
colonies grown under any conditions. The 16S rRNA gene is amplified
using universal primers and thermalcyclers. The amplified 16S
rRNA gene product is sequenced using dye terminator cycle sequencing
chemistry. The sequence reactions are analyzed using automated
DNA sequencers and software. Unknown bacterial samples are identified
using the MicroSeq™ microbial identification software and
16S rRNA gene sequence database, containing over 1,500 entries.
Dependant upon the level of confidence, speed and cost desired,
either the entire 1540 base pair 16S rRNA gene, or a smaller portion
of the gene can be (500 bp) sequenced. A printout of the actual
sequence data and the identification is included in each report. |
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Address
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Microbe Inotech Labs, Inc. |
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12133 Bridgeton Square Drive
St. Louis
MO
USA |
| Tel. |
+1 (800) 688 9144 |
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